1P032 Structural study on fluorescent properties of new GFP-like proteins

GFP-like proteins stably accumulate in lysosomes.

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, its GFP variants (Aequorea GFPs), and more recently the novel GFP-like proteins from Anthozoa have greatly advanced our technologies for fluorescently labeling cells, organelles, and proteins. It has been shown, however, that some GFP-like proteins have a tendency to oligomerize and aggregate. Transfection of GFP-like protein...

X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein

Phanta is a reversibly photoswitching chromoprotein (ΦF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (ΦF, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combination, to the optical properties of Phanta. Single amino acid substitutions at position 193 resulte...

Alternative Cyclization in GFP-like Proteins Family

Photoactivated structural dynamics of fluorescent proteins.

Proteins of the GFP (green fluorescent protein) family have revolutionized life sciences because they allow the tagging of biological samples in a non-invasive genetically encoded way. 'Phototransformable' fluorescent proteins, in particular, have recently attracted widespread interest, as their fluorescence state can be finely tuned by actinic light, a property central to the development of su...

Usefulness of Fluorescent Proteins: Gfp, Dsred and Fruit Fluorescent Proteins Inidentifying Multisynaptic Neuronal Chains. History and Our Own Experience

Investigation of the neuronalconnectionshave been conducted over a long span of time. Debate between researchers about contiguity or continuity of the nerve elements resulted in a new era in the research of neuronal pathways. The issue has been resolved when synaptic connections were discovered by the electron microscope. This made it possible to use two types of tract tracing methods. First no...